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t denticola atcc 35405 wild type strain  (ATCC)


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    ATCC t denticola atcc 35405 wild type strain
    T Denticola Atcc 35405 Wild Type Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 760 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 98 stars, based on 760 article reviews
    t denticola atcc 35405 wild type strain - by Bioz Stars, 2026-03
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    Inducible expression of Msp in a complemented T. denticola Δ msp mutant. T. denticola CF1090 (CF637 Δ msp ) was transformed with shuttle plasmid pCF1173 (A), which carries msp under transcriptional control of the tetracycline-inducible promoter derived from pRPF185 (B), yielding a complemented T. denticola Δ msp mutant strain CF1179. Expression of Msp in T. denticola parent <t>ATCC</t> <t>35405</t> and complemented Δ msp mutant CF1179 was assayed by western immunoblots probed with antibodies raised against native Msp (C) and FlaA1 (D). CF1179 was grown in concentrations of ATc ranging from 0 to 100 ng/mL to induce Msp expression. Samples probed with anti-Msp were held on ice (u) or boiled (b) prior to electrophoresis to detect Msp in monomers and oligomers (arrows), respectively.
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    Isogenic T Denticola Atcc 35405 Derived Strains Cf734, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    wild type strain n a n a atcc cf543  (ATCC)
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    Wild Type Strain N A N A Atcc Cf543, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    t denticola strain atcc 35405 coaggregate  (ATCC)
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    ATCC t denticola strain atcc 35405 coaggregate
    Inducible expression of Msp in a complemented T. denticola Δ msp mutant. T. denticola CF1090 (CF637 Δ msp ) was transformed with shuttle plasmid pCF1173 (A), which carries msp under transcriptional control of the tetracycline-inducible promoter derived from pRPF185 (B), yielding a complemented T. denticola Δ msp mutant strain CF1179. Expression of Msp in T. denticola parent <t>ATCC</t> <t>35405</t> and complemented Δ msp mutant CF1179 was assayed by western immunoblots probed with antibodies raised against native Msp (C) and FlaA1 (D). CF1179 was grown in concentrations of ATc ranging from 0 to 100 ng/mL to induce Msp expression. Samples probed with anti-Msp were held on ice (u) or boiled (b) prior to electrophoresis to detect Msp in monomers and oligomers (arrows), respectively.
    T Denticola Strain Atcc 35405 Coaggregate, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t denticola strain atcc 35405 coaggregate/product/ATCC
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    Inducible expression of Msp in a complemented T. denticola Δ msp mutant. T. denticola CF1090 (CF637 Δ msp ) was transformed with shuttle plasmid pCF1173 (A), which carries msp under transcriptional control of the tetracycline-inducible promoter derived from pRPF185 (B), yielding a complemented T. denticola Δ msp mutant strain CF1179. Expression of Msp in T. denticola parent ATCC 35405 and complemented Δ msp mutant CF1179 was assayed by western immunoblots probed with antibodies raised against native Msp (C) and FlaA1 (D). CF1179 was grown in concentrations of ATc ranging from 0 to 100 ng/mL to induce Msp expression. Samples probed with anti-Msp were held on ice (u) or boiled (b) prior to electrophoresis to detect Msp in monomers and oligomers (arrows), respectively.

    Journal: Molecular oral microbiology

    Article Title: Expanded Functional Characterization and Optimization of Protein Expression in Treponema denticola Shuttle Plasmids

    doi: 10.1111/omi.70016

    Figure Lengend Snippet: Inducible expression of Msp in a complemented T. denticola Δ msp mutant. T. denticola CF1090 (CF637 Δ msp ) was transformed with shuttle plasmid pCF1173 (A), which carries msp under transcriptional control of the tetracycline-inducible promoter derived from pRPF185 (B), yielding a complemented T. denticola Δ msp mutant strain CF1179. Expression of Msp in T. denticola parent ATCC 35405 and complemented Δ msp mutant CF1179 was assayed by western immunoblots probed with antibodies raised against native Msp (C) and FlaA1 (D). CF1179 was grown in concentrations of ATc ranging from 0 to 100 ng/mL to induce Msp expression. Samples probed with anti-Msp were held on ice (u) or boiled (b) prior to electrophoresis to detect Msp in monomers and oligomers (arrows), respectively.

    Article Snippet: To further characterize shuttle plasmid-mediated complementation in T. denticola ATCC 35405, we used several different promoter/gene of interest combinations in complementation studies of T. denticola ATCC 35405 mutant strains ( and ).

    Techniques: Expressing, Mutagenesis, Transformation Assay, Plasmid Preparation, Control, Derivative Assay, Western Blot, Electrophoresis